Thermo Scientific™ GeneBLAzer™ LXRα-UAS-bla HEK 293T Cells

Description

LXR alpha-UAS-bla HEK 293T 293 cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-alpha(LXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

LXR alpha-UAS-bla HEK 293T 293 cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-alpha(LXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-LXR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. LXR alpha-UAS-bla HEK 293T 293 cells are functionally validated for Z' and EC₅₀ concentrations of TO901317. In addition, LXR alpha-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

• Alternate Agonists: Cholic Acid, Deoxycholic Acid
• Antagonists: (z)-guggulsterone
• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Dividing Cells
• Content And Storage: LXR alpha-UAS-bla HEK 293T cells (K1249)
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen)
• Detection Method: Fluorescence
• Species: Human
• Druggable Target: Nuclear Receptors
• No. of Cells: 2 x 10⁶
• Primary Agonist: Chenodeoxycholic Acid (CDCA)
• Product Line: GeneBLAzer™
• Product Type: HEK 293T Cells
• Quantity: 2 x 10⁶ cells
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: LXRA, LXR alpha, NR1H3

For Research Use Only. Not for use in diagnostic procedures.