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Thermo Scientific™ GeneBLAzer™ PPARδ-UAS-bla HEK 293T Cells

Catalog No. K1690
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Quantity:
2 x 106 cells

Description

PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR delta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PPAR delta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of L-165041. In addition, PPAR delta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists Estriol, Estrone, Genistein, Diethylstibestrol, Ethynylestradiol
Antagonists RU486+1 nM E2, 4-OH-tam+1 nM E2, 4-OH-tamoxifen, Tamoxifen, Tam+1 nM E2, RU486
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage PPAR delta-UAS-bla HEK 293T cells (K1244)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Detection Method Fluorescence
Species Human
Druggable Target Nuclear Receptors
No. of Cells 2 x 106
Primary Agonist 17-β-estradiol
Product Line GeneBLAzer™
Product Type HEK 293T Cells
Quantity 2 x 106 cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry PPAR delta, NR1C2, PPARD
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For Research Use Only. Not for use in diagnostic procedures.

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