MP Biomedicals™ GeneClean II Kit

GeneClean™ II Kit

Manufacturer:  MP Biomedicals™ 111001400

Catalog No. 11-100-1400

Description

• The GENECLEAN™ II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions. Glassmilk™ slurry allows flexibility of reacion size and volume.
• The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.
• The GENECLEAN™ II Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels.
• This kit can also be used to purify DNA from polyacrylamide gels.
• Typical recovery of DNA is up to 80%.
• Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.
• The GENECLEAN™ II Kit combines silica-binding technology with the convenience of a spin or vacuum column format.
• DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane.
• Contaminants are removed by a simple spin or vacuum wash.
• The bound DNA is then eluted.
• DNA fragments of interest are extracted by solubilizing slices of an agarose gel.
• The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer.
• The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution.
• Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.

Specifications

• For Use With (Application): Nucleic Acid Isolation & Purification
• Format: Solution orgels
• Product Type: Kits & Assays
• Storage Requirements: All gENECLEAN™ Kits are shipped at room temperature (15-30°C).

• Concentration: New Wash/Ethanol Concentration must be ∽ 50%
• pH: Above 500 bp: 4.5 M NaI pH 7.0 - 7.4; Between 200 - 500 bp: 4.5 M NaI pH 6.0; NEW Wash: pH Range: 7.4 - 8.0
• Quantity: 300 preps
• Synonym: Rapid purification of linear DNA