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MP Biomedicals™ GeneClean II Kit Available on GSA/VA Contract for Federal Government customers only.

GeneClean® II Kit

Manufacturer:  MP Biomedicals™ 111001400

Catalog No. 11-100-1400


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Description

Description

  • The GENECLEAN® II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions. Glassmilk™ slurry allows flexibility of reacion size and volume.
  • The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.
  • The GENECLEAN® II Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels.
  • This kit can also be used to purify DNA from polyacrylamide gels.
  • Typical recovery of DNA is up to 80%.
  • Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.
  • The GENECLEAN® II Kit combines silica-binding technology with the convenience of a spin or vacuum column format.
  • DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane.
  • Contaminants are removed by a simple spin or vacuum wash.
  • The bound DNA is then eluted.
  • DNA fragments of interest are extracted by solubilizing slices of an agarose gel.
  • The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer.
  • The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution.
  • Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.

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Specifications

Specifications

New Wash/Ethanol Concentration must be ∽ 50%
Solution orgels
Kits & Assays
AllgENECLEAN® Kits are shipped at room temperature (15-30°C).
Nucleic Acid Isolation & Purification
Above 500 bp: 4.5 M NaI pH 7.0 - 7.4; Between 200 - 500 bp: 4.5 M NaI pH 6.0; NEW Wash: pH Range: 7.4 - 8.0
300 preps
Rapid purification of linear DNA
Documents
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