MP Biomedicals™ GeneClean II Kit
GeneClean™ II Kit
Manufacturer: MP Biomedicals™ 111001400
- The GENECLEAN™ II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions. Glassmilk™ slurry allows flexibility of reacion size and volume.
- The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.
- The GENECLEAN™ II Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels.
- This kit can also be used to purify DNA from polyacrylamide gels.
- Typical recovery of DNA is up to 80%.
- Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.
- The GENECLEAN™ II Kit combines silica-binding technology with the convenience of a spin or vacuum column format.
- DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane.
- Contaminants are removed by a simple spin or vacuum wash.
- The bound DNA is then eluted.
- DNA fragments of interest are extracted by solubilizing slices of an agarose gel.
- The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer.
- The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution.
- Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.
|Nucleic Acid Isolation & Purification|
|Kits & Assays|
|All gENECLEAN™ Kits are shipped at room temperature (15-30°C).|
|New Wash/Ethanol Concentration must be ∽ 50%|
|Above 500 bp: 4.5 M NaI pH 7.0 - 7.4; Between 200 - 500 bp: 4.5 M NaI pH 6.0; NEW Wash: pH Range: 7.4 - 8.0|
|Rapid purification of linear DNA|
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