Thermo Scientific™ GeneJET RNA Purification Kit

Description

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights

• Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient—high yields of total RNA
• Fast—15 minutes after lysis step
• Pure—an A260/280 ratio > 1.9
• Convenient—spin columns are capped and assembled with collection tubes

Applications

Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:

• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Includes

Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol

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Specifications

• Content And Storage: • 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening
  • 200 mL Lysis Buffer; room temperature
  • 200 mL Wash Buffer 1 (concentrated); room temperature
  • 100 mL Wash Buffer 2 (concentrated); room temperature
  • 125 mL Nuclease-free Water; room temperature
  • 250 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
  • 250 Collection Tubes (2 mL); room temperature
  • 250 Collection Tubes (1.5 mL); room temperature
• Isolation Technology: Silica Spin Column
• Purification Time: 15 min. (After sample homogenization)
• Elution Volume: 50–100 μL
• Sample Type: Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
• Final Product Type: Total RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Quantity: 250 Preps
• Scale: Mini
• Shipping Condition: Room Temperature
• Starting Material Amount: Bacteria: ≤10⁹ cells, Blood: 0.5 mL, Cells: ≤10⁷, Tissue and Insects: 30 mg, Yeast: ≤4 x 10⁸ cells, Enzymatic reactions: ≤100 μL
• Yield: Up to 160 μg

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.