Thermo Scientific™ GeneJET RNA Purification Kit

Silica membrane-based total RNA purification from mammalian blood, cell cultures, and tissues as well as insect, yeast, and bacteria. Provided in a convenient spin-column format.

Catalog Number	Mfr. No.	No. of Reactions
FERK0732	Thermo Scientific™ K0732	250 Preps
FERK0731	Thermo Scientific™ K0731	50 Preps

Description

GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights

• Universal– can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient– high yields of total RNA.
• Fast– 15 minutes after lysis step
• Pure– an A260/280ratio > 1.9
• Convenient– spin columns are capped and assembled with collection tubes

Applications

• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
  • RT-PCR
  • RT-qPCR
  • Northern blotting
  • Nuclease protection assay

• Proteinase K
• Lysis Buffer
• Wash Buffer 1 (concentrated)
• Wash Buffer 2 (concentrated)
• Water, nuclease-free
• GeneJETRNA Purification Columns (pre-assembled with Collection Tubes)
• CollectionTubes(2ml)
• Collection Tubes (1.5ml)
• Detailed Protocol

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Specifications

• Format: Spin Column
• Purification Time: 15 min.
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: GeneJET

• Isolation Technology: Spin Column
• Sample Type: Bacteria, Blood, Cells, Tissue, Yeast
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Northern Blotting, cDNA Library Construction
• Target: Total RNA