Thermo Scientific™ GeneJET Stabilized and Fresh Whole Blood RNA Kit

Description

Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.

The GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).

Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).

Highlights

• Versatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes
• Convenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
• Efficient—up to 4 μg of total RNA from 500 μL of healthy human blood
• Pure—isolates intact total RNA free of any RT-qPCR inhibitors

Applications

Isolated total RNA can be used in:

• RT-PCR
• RT-qPCR
• Northern Blotting

Includes

• Lysis Buffer (R2841)
• Wash Buffer 1 (conc.)(R2851)
• Wash Buffer 2 (conc.)(R2861)
• DNase l (lyophilized)
• DNase I Reconstitution Buffer
• 2x DNase I Buffer
• Manganese Chloride Solution
• Water, nuclease-free
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes
• Collection Tubes, 2 mL
• Collection Tubes, 1.5 mL

Specifications

• Concentration: 2X
• Content And Storage: For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
• Format: Spin Column
• Isolation Technology: Spin Column
• Purification Time: 30 min.
• Sample Type: Blood
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Northern Blotting, cDNA Library Construction
• No. of Reactions: 50 Preps
• Product Line: GeneJET
• Quantity: 50 preps
• Target: Total RNA

For Research Use Only. Not for use in diagnostic procedures.