Invitrogen™ Human Ghrelin ELISA Kit

Catalog No.	Quantity
50-112-5359	96 Tests

Catalog No. 50-112-5359 Supplier Invitrogen™ Supplier No. BMS2192

Description

ELISA

The Human Ghrelin ELISA quantitates Hu Ghrelin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Ghrelin. Principle of the method The Human Ghrelin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an the ligand for the growth hormone secretagogue receptor type 1 and is involved in regulating growth hormone release. Ghrelin has an appeptite-stimulating effect and induces adiposity and stimulates gastric acid secretion. Obestatin may be the ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and can have an appetite-reducing effect resulting in decreased food intake.

Specifications

• Accession Number: Q9UBU3
• Assay Range: 15.6-1000.0 pg/mL
• Assay Sensitivity: 11.8 pg/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human Ghrelin
  Biotin-Conjugate anti-human Ghrelin monoclonal antibody
  Streptavidin-HRP
  Human Ghrelin Standard lyophilized, 2000 pg/mL upon reconstitution
  Sample Diluent
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Adhesive Films
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 2210006E23Rik; AMCBX1; Appetite-regulating hormone; appetite-regulating hormone; uncharacterized protein LOC58991; exon 2- and 3-deleted preproghrelin; exon 2-deleted preproghrelin; FIP3; FIP-3; Fip3p; Ghr; Ghrelin; ghrelin 27; ghrelin 28; ghrelin and obestatin prepropeptide; ghrelin precursor; ghrelin, growth hormone secretagogue receptor ligand; ghrelin/obestatin; ghrelin/obestatin preprohormone; ghrelin/obestatin prepropeptide; Ghrelin-27; Ghrelin-28; ghrelin-like protein; GHRL; Growth hormone secretagogue; growth hormone-releasing peptide; IKK-gamma; In2c-preproghrelin; IP; IP1; IP2; IPD2; m46; Motilin-related peptide; MTLRP; MTLRPAP; NEMO; Obestatin; Obestatin-13; Obestatin-23; OTTHUMP00000207794; OTTHUMP00000207795; OTTHUMP00000207796; OTTHUMP00000207798; prepro-appetite regulatory hormone; preproghrelin; proghrelin; Protein M46; UNQ524/PRO10662210006E23Rik; AMCBX1; Appetite-regulating hormone; appetite-regulating hormone; uncharacterized protein LOC58991; exon 2- and 3-deleted preproghrelin; exon 2-deleted preproghrelin; FIP3; FIP-3; Fip3p; Ghr; Ghrelin; ghrelin 27; ghrelin 28; ghrelin and obestatin prepropeptide; ghrelin precursor; ghrelin, growth hormone secretagogue receptor ligand; ghrelin/obestatin; ghrelin/obestatin preprohormone; ghrelin/obestatin prepropeptide; Ghrelin-27; Ghrelin-28; ghrelin-like protein; GHRL; Growth hormone secretagogue; growth hormone-releasing peptide; IKK-gamma; In2c-preproghrelin; IP; IP1; IP2; IPD2; m46; Motilin-related peptide; MTLRP; MTLRPAP; NEMO; Obestatin; Obestatin-13; Obestatin-23; OTTHUMP00000207794; OTTHUMP00000207795; OTTHUMP00000207796; OTTHUMP00000207798; prepro-appetite regulatory hormone; preproghrelin; proghrelin; Protein M46; UNQ524/PRO1066; MTLRP
• Gene ID (Entrez): 51738
• Gene Symbol: GHRL
• Interassay CV: 8.5%
• Intraassay CV: 6%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 25 μL, Serum 25 μL, Supernatant 25 μL
• Storage Requirements: 2-8°C
• Synonym: GHRL ghrelin
• Target: Ghrelin
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min