Gibco™ Advanced DMEM

Catalog No.	Quantity
12-491-015	500 mL
12-491-023	10 x 500 mL

Catalog No. 12-491-015 Supplier Gibco™ Supplier No. 12491015

Description

Requires

Supplementation

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

This Advanced DMEM is manufactured as follows:

With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine

The complete formulation is available.

Using Advanced DMEM

Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Line: MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
• Classification: Animal Origin
• Concentration: 1 X
• Form: Liquid
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)
• Serum Level: Reduced Serum
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
• Without Additives: No Glutamine, No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

For Research Use Only. Not for use in diagnostic procedures.