Gibco™ Advanced DMEM
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
Supplier: Gibco™ 12491023
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.
This Advanced DMEM is manufactured as follows:
With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
The complete formulation is available.
Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Shipping Condition: Room Temperature
|Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
|High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)|
|MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero|
|DMEM (Dulbecco's Modified Eagle Medium)|
|No Glutamine, No HEPES|
|cGMP-compliant under the ISO 13485 standard|
|10 x 500 mL|
Safety and Handling
ShelfLife : 12 Months From Date of Manufacture
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For Research Use Only. Not for use in diagnostic procedures.