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Gibco™ Advanced DMEM

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Supplier:  Gibco™ 12491023


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Catalog No. 12-491-023

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Requires : Supplementation



Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

This Advanced DMEM is manufactured as follows:
With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine

The complete formulation is available.

Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature



Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Animal Origin
Reduced Serum
High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Sustainable packaging
Room Temperature
MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
1 X
DMEM (Dulbecco's Modified Eagle Medium)
No Glutamine, No HEPES
cGMP-compliant under the ISO 13485 standard
10 x 500 mL
Safety and Handling

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture



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For Research Use Only. Not for use in diagnostic procedures.