Advanced MEM, HEPES Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Advanced Minimum Essential medium is widely used basal medium that allows culture of mammalian cells with reduced Fetal Bovine Serum supplementation

Manufacturer: Gibco 12492013

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Catalog No. 12-492-013

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Required Accessories : Supplementation


    Format Liquid
    Formulation AlbuMAX II, Ammonium Metavanadate, Ascorbic Acid phosphate, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, Folic Acid, Glycine, Human Transferrin (Holo), Insulin Recombinant Full Chain, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4-H2O), Thiamine hydrochloride, i-Inositol
    Packaging Sustainable packaging
    Quantity 500mL
    Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
    With Additives Sodium Pyruvate; Low Glucose; No Glutamine; Phenol Red

    Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2. It is modified as follows: with glucose, non essential amino acids, sodium pyruvate, phenol red; without L-glutamine, HEPES.

    • Unique from other media due to addition of following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride
    • Requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement
    • Many cell lines do not require adaptation to this media
    • FBS concentration must be optimized for each cell line to obtain maximum serum reduction
    • Uses sodium bicarbonate buffer system (3.7g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
    • Sustainable packaging

    Cell Culture, Mammalian Cell Culture

  • Description & Specifications

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