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Gibco™ Advanced MEM
GSA_VA

Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Manufacturer:  Gibco™ 12492013

Encompass

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Catalog No. 12-492-013


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Description

Description

  • Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation
  • Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology
  • Advanced MEM is includes following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride
  • Requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement
  • Many cell lines do not require adaptation to this media
  • The FBS concentration must be optimized for each cell line to obtain maximum serum reduction
  • Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2
  • Store at 2°C to 8°C, protected from light

Modified as follows:

  • With: Glucose, non-essential amino acids, sodium pyruvate, and phenol red
  • Without: L-glutamine, HEPES

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Specifications

Specifications

1X
Sterile-filtered
Advanced (Reduced Serum)
Gibco™
No Glutamine
12 Months From Date of Manufacture
500 mL
HEPES, L-Glutamine
Sodium Pyruvate
Animal Origin
No HEPES
MEM (Minimum Essential Medium)
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Ambient
Liquid
Phenol Red
Sustainable packaging
Glucose, Non Essential Amino Acids, Phenol Red, Sodium Pyruvate
Low Glucose
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
MEM (Minimum Essential Medium)
SDS
Documents
Product Certifications
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For Research Use Only. Not for use in diagnostic procedures.