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Gibco™ Advanced MEM Non-distribution product as customer accommodation. Greener Choice Available on GSA/VA Contract for Federal Government customers only.

Advanced Minimum Essential medium is widely used basal medium that allows culture of mammalian cells with reduced Fetal Bovine Serum supplementation

Manufacturer:  Gibco™ 12492013

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Catalog No. 12-492-013


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Requires : Supplementation

Description

Description

Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2. It is modified as follows: with glucose, non essential amino acids, sodium pyruvate, phenol red; without L-glutamine, HEPES.

  • Unique from other media due to addition of following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride
  • Requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement
  • Many cell lines do not require adaptation to this media
  • FBS concentration must be optimized for each cell line to obtain maximum serum reduction
  • Uses sodium bicarbonate buffer system (3.7g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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Specifications

Specifications

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
No HEPES
500mL
Room Temperature
500 ml
No Glutamine
Low Glucose
Gibco®
Advanced (Reduced Serum)
Sodium Pyruvate
Liquid
Phenol Red
SDS
Documents
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For Research Use Only. Not for use in diagnostic procedures.