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Gibco™ Agarose (4%)
Description
- The 4% Agarose Gel melts at 65 to 70°C and remains fluid at 37°C
- When diluted with concentrated insect cell culture medium, Gibco™ 4% Agarose produces a gel firm enough to immobilize local infections, yet ductile enough to allow for the efficient removal of plaques for clonal expansion
Cell Culture, Mammalian Cell Culture
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | Storage conditions: 2 – 8°C. Protect from light. Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Cell Type | Mammalian Cells |
| Concentration | 0.04 |
| Form | Gel |
| Product Type | Agarose |
| Sterility | Sterile |
| Sterilization Method | Autoclaved |
| For Use With (Application) | Plaque Assay |
| Quantity | 40 mL |
| Remarks | Not for electrophoresis |
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Frequently Asked Questions (FAQs)
No. Autoclaving at temperatures that are too high or pressures that are too high can hydrolyze the agarose polymer and weaken it.
Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.
The agarose is reconstituted in distilled water.
For Research Use Only. Not for use in diagnostic procedures.
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