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Gibco™ Agarose (4%)

Catalog No. 18300012
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Catalog No. Quantity
18-300-012 40 mL
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Catalog No. 18-300-012 Supplier Gibco™ Supplier No. 18300012
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An autoclaved, high purity, low melting point gel designed for plaque assays and the purification of baculovirus

  • The 4% Agarose Gel melts at 65 to 70°C and remains fluid at 37°C
  • When diluted with concentrated insect cell culture medium, Gibco™ 4% Agarose produces a gel firm enough to immobilize local infections, yet ductile enough to allow for the efficient removal of plaques for clonal expansion

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Type Mammalian Cells
Concentration 0.04
Form Gel
Product Type Agarose
Sterility Sterile
Sterilization Method Autoclaved
For Use With (Application) Plaque Assay
Quantity 40 mL
Remarks Not for electrophoresis
Shipping Condition Room Temperature
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Can I autoclave agarose to prepare agarose gels?

No. Autoclaving at temperatures that are too high or pressures that are too high can hydrolyze the agarose polymer and weaken it.

How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?

Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.

For Agarose (4%) (Cat. No. 18300012), what is the agarose reconstituted in?

The agarose is reconstituted in distilled water.

For Research Use Only. Not for use in diagnostic procedures.

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