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Gibco™ DMEM/F-12 Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only. Green Product

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM/F-12 is used in cell culture processing applications.

Manufacturer:  Gibco™ 11320082

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Catalog No. 11-320-082


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Description

Description

  • Contains four times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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Specifications

Specifications

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Gibco®
Standard Serum Supplementation
Sodium Pyruvate
Liquid
Phenol Red
High Glucose
10 x 500mL
Room Temperature
500 ml
L-Glutamine
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