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  7. Gibco™ DMEM/F-12, powder 

Gibco™ DMEM/F-12, powder

Catalog No. p-4919851
Encompass_Preferred
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Quantity:
50 L
10 x 1 L
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.

Using DMEM/F-12
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Classification Animal Origin-free
Form Powder
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Quantity 10 x 1 L
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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