Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Gibco™ DMEM/F-12, powder

Catalog No. p-4919851
Encompass_Preferred
Click to view available options
Quantity:
50 L
10 x 1 L
This item is not returnable. View return policy
This item is not returnable. View return policy

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.

Using DMEM/F-12
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Classification Animal Origin-free
Form Powder
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Quantity 10 x 1 L
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Show More Show Less
WARNING: Cancer - www.P65Warnings.ca.gov
Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.