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Gibco™ DMEM/F-12, powder

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

$87.25 - $232.00

Specifications

Content And Storage Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Form Powder
Serum Level Standard Serum Supplementation
With Additives High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives No HEPES
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
12-500-062
Encompass_Preferred
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Gibco™
12500062
10 x 1 L
Each for $87.25
Edge
Add to Cart
 
12-500-096
Encompass_Preferred
View Documents
Gibco™
12500096
50 L
Each for $232.00
Add to Cart
 
Description

Description

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.

Using DMEM/F-12
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Standard Serum Supplementation
No HEPES
Gibco™
Powder
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Room Temperature
Videos
Safety and Handling

Safety and Handling

ShelfLife : 36 Months From Date of Manufacture

SDS
Documents

Documents

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