Gibco™ DMEM/F-12, powder, HEPES

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Supplier:  Gibco™ 12400024

Catalog No. 12-400-024

Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:
With: L-glutamine, HEPES, Phenol Red
Without: Sodium Bicarbonate

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

• Content And Storage: Storage conditions: 2-8°C
  Shipping conditions: Ambient
  Shelf life: 36 months from date of manufacture
• Classification: Animal Origin-free
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
• Sterility: Sterile-filtered
• Without Additives: No Sodium Bicarbonate
• Product Line: Gibco™
• Shipping Condition: Room Temperature

• Cell Line: MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
• Form: Powder
• Serum Level: Standard Serum Supplementation
• With Additives: High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 10 x 1 L

Safety and Handling

ShelfLife : 36 Months From Date of Manufacture

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