Gibco™ DMEM, high glucose

Description

Requires

Supplementation

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red

Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Line: HeLa, 293, Cos-7, and PC-12
• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Classification: Animal Origin-free
• Concentration: 1 X
• Form: Liquid
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: High Glucose, Glutamine, Phenol Red
• Without Additives: No HEPES, No Sodium Pyruvate
• For Use With (Application): Mammalian Cell Culture
• Glucose Concentration: 4500 mg/L
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco™
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2°C to 8°C (protect from light)
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.