Gibco™ DMEM, high glucose, HEPES, no phenol red

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, HEPES, No Phenol Red is used in cell culture processing applications.

Supplier:  Gibco™ 21063045

Catalog No. 21-063-045

Description

• Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
• Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO₂ environment to maintain physiological pH
• Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Order Info

Shipping Condition: Room Temperature

Compliance

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Concentration: 1 X
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)
• Shelf Life: 12 Months From Date of Manufacture
• With Additives: High Glucose, Glutamine, HEPES
• Green Features: Sustainable packaging
• Quantity: 10 x 500 mL

• Cell Line: HeLa, 293, Cos-7, and PC-12
• Classification: Animal Origin-Free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• Without Additives: No Phenol Red, No Sodium Pyruvate
• Product Line: Gibco™
• Shipping Condition: Room Temperature