Gibco™ DMEM, high glucose, NEAA, no glutamine   

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, NEAA, No Glutamine, Sodium Bicarbonate Buffer is used in human ex vivo tissue and cell culture processing applications.

Manufacturer:  Gibco™ 10938025

Catalog No. 10-938-025

Description

• Animal-origin free
• Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
• Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO₂ environment to maintain physiological pH
• Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco DMEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

• Classification: Animal Origin-Free
• Content And Storage: Storage conditions: 2-8° C. Protect from light.
  Shipping conditions: Ambient.
  Shelf life: 12 months from date of manufacture.
• Form: Liquid
• Glutamine: No Glutamine
• HEPES Buffer: No HEPES
• Phenol Red Indicator: Phenol Red
• Serum Supplementation: Standard Serum Supplementation
• Sodium Bicarbonate Buffer: Sodium Bicarbonate
• Supplementation Required: Serum
• pH Range: 7.0 - 7.2

• Concentration: 1 X
• Culture Environment: CO2
• Glucose: High Glucose
• Green Features: Sustainable packaging
• Osmolality: 310 - 360 mOsm/kg
• Product Line: Gibco™
• Shipping Condition: Room Temperature
• Sodium Pyruvate Additive: No Sodium Pyruvate
• Volume (Metric): 500 ml

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.