Gibco™ DMEM, High Glucose, NEAA, No Glutamine, Sodium Bicarbonate Buffer   

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, NEAA, No Glutamine, Sodium Bicarbonate Buffer is used in human ex vivo tissue and cell culture processing applications.

Manufacturer:  Gibco™ 10938025

Catalog No. 10-938-025

Description

• Animal-origin free
• Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
• Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO₂ environment to maintain physiological pH
• Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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Order Info

Shipping Condition: Room Temperature

Compliance

Gibco DMEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

• Concentration: 1X
• Format: Liquid
• Packaging: Sustainable packaging
• Product Type: DMEM media
• Storage Requirements: Storage: 2°C to 8°C. Protect from light.
• Without Additives: L-glutamine, Sodium Pyruvate, HEPES

• For Use With (Application): Mammalian cell culture
• Formulation: Calcium Chloride (CaCl₂-2H₂O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO₃)₃·9H₂O), Folic Acid, Glycine, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H₂O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO₄-7H₂O), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO₃), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH₂PO₄-2H₂O), Thiamine hydrochloride, i-Inositol
• pH: 7.0 - 7.2
• Quantity: 500mL
• With Additives: High Glucose, Non Essential Amino Acids, Phenol Red

Safety and Handling

ShelfLife : 12 months from date of manufacture

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.