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Gibco™ DMEM, High Glucose, NEAA, No Glutamine, Sodium Bicarbonate Buffer Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only. Green Product

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, NEAA, No Glutamine, Sodium Bicarbonate Buffer is used in human ex vivo tissue and cell culture processing applications.

Manufacturer:  Gibco™ 10938025

Catalog No. 10-938-025


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Description

Description

  • Animal-origin free
  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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Order Info

Shipping Condition: Room Temperature

Compliance

Gibco DMEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

Specifications

1X
Liquid
Sustainable packaging
DMEM media
Storage: 2°C to 8°C. Protect from light.
L-glutamine, Sodium Pyruvate, HEPES
Mammalian cell culture
Calcium Chloride (CaCl2-2H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-2H2O), Thiamine hydrochloride, i-Inositol
7.0 - 7.2
500mL
High Glucose, Non Essential Amino Acids, Phenol Red
Safety and Handling

Safety and Handling

ShelfLife : 12 months from date of manufacture

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For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.