Gibco DMEM, High Glucose, No Glutamine
Used as basal medium for supporting the growth of many different mammalian cells. Gibco DMEM, High Glucose, No Glutamine is used in cell culture processing applications.
Manufacturer: Gibco 11960051
- Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
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|Mammalian Cell Culture|
|Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol|
|Storage: 2-8° C. Protect from light|
|L-glutamine; Sodium Pyruvate; HEPES|
|High Glucose; Phenol Red|
Safety and Handling
ShelfLife : 12 months from date of manufacture
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When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.