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Gibco™ DMEM, powder, high glucose
GSA_VA

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, Powder, High Glucose, No Sodium Bicarbonate Buffer is used in cell culture processing applications.

Manufacturer:  Gibco™ 12100046

Encompass

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Catalog No. 12-100-046


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Description

Description

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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Specifications

Specifications

DMEM (Dulbecco's Modified Eagle Medium)
Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Room Temperature
Gibco™
L-Glutamine
36 Months From Date of Manufacture
primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
HEPES, Sodium Pyruvate, Sodium Bicarbonate
No Sodium Pyruvate
Animal Origin-Free
No HEPES
Sterile-filtered
Standard Serum Supplementation
No Sodium Bicarbonate
Powder
Phenol Red
10 x 1L
High Glucose, L-Glutamine, Phenol Red
High Glucose
HeLa, 293, Cos-7, and PC-12
DMEM (Dulbecco's Modified Eagle Medium)
SDS
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In vitro diagnostic