Gibco™ EBSS, no calcium, no magnesium, no phenol red

Catalog No.	Quantity
14-155-063	500 mL

Catalog No. 14-155-063 Supplier Gibco™ Supplier No. 14155063

Description

Earle's Balanced Salt Solution (EBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Earle's Balanced Salt Solution (EBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Gibco™ EBSS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.

This EBSS is manufactured as follows:

With: Glucose, Sodium Bicarbonate

Without: Calcium, Magnesium, Phenol Red

The complete formulation is available.

Order Info

Shipping Condition: Room Temperature

Specifications

• Chemical Name or Material: Earle's Balanced Salt
• Concentration: 1 X
• For Use With (Application): Variety of Cell Culture Applications; Such as Washing Cells Before Dissociation; Transporting Cells or Tissue Samples; Diluting Cells for Counting; and Preparing Reagents
• pH: 7.0 - 8.1
• Physical Form: Liquid
• Product Line: Gibco
• Quantity: 500 mL
• Solution Type: Earle's Balanced Salt
• Sterility: Sterile-filtered
• Includes: No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Osmolality: 270 - 310 mOsm/kg

Frequently Asked Questions (FAQs)

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.