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Gibco™ Expi293™ Expression System Kit
Description
The Expi293 Expression System is a complete, scalable platform for rapid, high-yield transient protein production from mammalian HEK293 cells. The system includes: high-density Expi293F cells in Expi293 Expression Medium and ExpiFectamine 293 transfection reagent optimized with enhancers to maximize protein production and reproducibility.
- Complete kit—contains all components needed for efficient, recombinant protein expression with easy-to-follow culture and transfection protocols
- Up to 1 g/L of protein—2- to 10-fold higher protein yields than previous generation transient expression systems, such as the FreeStyle 293 Expression System
- Rapid production—rapid expression of recombinant proteins in just 2–7 days for a wide variety of protein formats from integral membrane proteins to monoclonal antibodies
- High density cultures—high-density Expi293F cultures yield significantly more protein-expressing cells per mL, boosting expression efficiency
- Native folding and mammalian post-translational modifications—proteins are expressed from human HEK293 cells resulting in a high degree of glycosylation and quality
- Scalable expression from <1 mL to >10 L—easily scalable and automation friendly from 96 deepwell plates for high throughput applications to multi-liter bioreactor platforms
The components included in the Expi293 Expression System Kit are: two vials of frozen Expi293F cells, one liter of Expi293 Expression Medium, one ExpiFectamine 293 Transfection kit sufficient to transfect up to 1 liter of final culture, Opti-MEM I Reduced Serum Medium complexation buffer, and an antibody-expressing positive control vector. Expi293 Transfection kits are also available in large-scale formats for transfecting up to 10 L and 50 L of Expi293 cells. Custom formats and volumes are also available upon request.
Engineered Expi293F cell lines are available for a range of structural biology applications, including Cryo-EM, NMR, and X-ray crystallography. Available individually or in kits.
- Expi293F GnTI- Cells (Cat. No. A39240)
- Expi293F Inducible Cells (Cat. No. A39241)
- Expi293F Inducible GnTI- Cells (Cat. No. A39242)
Specifications
Specifications
| Cell Line | Expi293F™ |
| Content And Storage | 2 vials frozen Expi293F cells 1 bottle 1000 mL Expi293 Expression Medium 1 ExpiFectamine 293 transfection kit for 1 L of culture 1 vial Antibody Expressing Positive Control Vector 1 bottle 100 mL Opti-MEM Reduced Serum Medium Store all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen |
| Delivery Type | Transfection |
| Cell Type | 293-derived Cells |
| Promoter | CMV |
| Transfection Technique | Lipid-based Transfection |
| Expression System | Mammalian |
| Expression Stability | Transient |
| For Use With (Application) | Protein Expression |
| Key Functions | Protein Production, Constitutive Expression |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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