Gibco™ Embryonic stem-cell FBS, qualified, USDA-approved regions

Catalog No.	Quantity	Format
10-439-001	50 mL	One Shot
10-439-024	500 mL	Bottle

Catalog No. 10-439-001 Supplier Gibco™ Supplier No. 10439001

Description

Embryonic stem-cell FBS, qualified, USDA-approved regions is US certified performance plus sera for broad range of cell types, especially sensitive cell lines.

Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

• Sera Category: Specialty
• Origin: Countries meeting USDA importation requirements
• Endotoxin level: ≤50 EU/mL (levels routinely ≤10 EU/mL)
• Hemoglobin level: ≤25 mg/dL

Specially tested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.

Order Info

Shipping Condition: Dry Ice

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Specifications

• Content And Storage: Storage conditions: ≤-10°C
  Shipping conditions: Frozen
• Species: Cattle/Bovine
• Age: Fetal
• Country of Origin: Mexico
• Form: Liquid
• Format: One Shot
• Product Type: Fetal Bovine Serum
• Quantity: 50 mL
• Serum Treatment: Standard (Sterile-filtered)
• Sterility: Sterile
• Tested For: Bovine Respiratory Syncytial Virus FA, Cytopathic Agents, Hemadsorbing Agents, Reovirus FA, Rabies FA, Hemoglobin (ng/dL), Blue Tongue Virus, Bovine Adenovirus FA, Bovine Parvovirus FA, Osmolality, Sp2 Performance Assay, Electrophoretic Pattern, Total Protein, Endotoxin (EU/mL), Mycoplasma, Sterility Testing (Bacteria, Fungi)
• Donor Herd: No
• Endotoxin Concentration: <50 EU/mL
• Hemoglobin Concentration: ≤25 mg/dL
• Purity or Quality Grade: ES Cell-Qualified
• Product Line: One Shot
• Shipping Condition: Frozen

Frequently Asked Questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Do I need to heat-inactivate Embryonic Stem-Cell FBS, qualified?

The material is not heat-inactivated. We would only recommend heat-inactivating it if your application specifically requires it. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.