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Gibco™ Glasgow's MEM (GMEM)
Description
Requires
Supplementation
Glasgow's MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle's Minimal Essential Medium. It was used to study the genetic factors that affected cell competence. Glasgow's MEM was developed for use with kidney cell lines, such as BHK-21.
This Glasgow's MEM is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Tryptose Phosphate Broth
The complete formulation is available.
Using Glasgow's MEM
Glasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins compared to the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth. Glasgow's MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow's MEM requires supplementation with 10% tryptose phosphate broth. Glasgow's MEM uses a sodium bicarbonate buffer system (2.75 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Cell Line | BHK-21 |
| Classification | Animal Origin-free |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | MEM (Minimum Essential Medium) |
| Sterility | Sterile-filtered |
| With Additives | High Glucose, Glutamine, Phenol Red |
| Without Additives | No HEPES, No Sodium Pyruvate, No Tryptose Phosphate Broth |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco™ |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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