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Gibco™ Glasgow's MEM (GMEM)
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Catalog No. 11710035
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11-710-035 500 mL
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Description

Requires

Supplementation

Glasgow's MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle's Minimal Essential Medium.

Glasgow's MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle's Minimal Essential Medium. It was used to study the genetic factors that affected cell competence. Glasgow's MEM was developed for use with kidney cell lines, such as BHK-21.

This Glasgow's MEM is modified as follows:

With: L-glutamine, Phenol Red

Without: HEPES, Tryptose Phosphate Broth

The complete formulation is available.

Using Glasgow's MEM

Glasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins compared to the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth. Glasgow's MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow's MEM requires supplementation with 10% tryptose phosphate broth. Glasgow's MEM uses a sodium bicarbonate buffer system (2.75 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Line BHK-21
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type MEM (Minimum Essential Medium)
Sterility Sterile-filtered
With Additives High Glucose, Glutamine, Phenol Red
Without Additives No HEPES, No Sodium Pyruvate, No Tryptose Phosphate Broth
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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