Gibco Grace's Insect Medium (2X), Supplemented, Lactalbumin Hydrolysate, Yeastolate
For use in the agarose overlay when performing the plaque assay
Manufacturer: Gibco 11667037
Grace's Supplemented Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Modified as follows: With Lactalbumin hydrolysate, Yeastolate, L-glutamine and Sodium bicarbonate. The complete formulation is available. Grace's Supplemented Insect Medium, 2x contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version commonly diluted with sterile water, Fetal bovine Serum and agarose before use in the agarose overlay, as described in the product manual.
Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture
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Shipping Condition: Room temperature
Gibco Grace's Supplemented Insect Medium, 2x is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
|Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture|
|Storage conditions: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture|
|Alpha-Ketoglutaric acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Fructose, D-Glucose (Dextrose), Folic Acid, Fumaric acid, Glycine, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Lactalbumin hydrolysate, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Malic acid, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Phosphate monobasic (Na2H2PO4-H2O), Succinic acid, Sucrose, Thiamine hydrochloride, Yeastolate, beta-Alanine, i-Inositol|
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For Research Use Only. Not for any animal or human therapeutic or diagnostic use.