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Gibco™ Ham's F-10 Nutrient Mix, HEPES

Ham's F-10 Nutrient Mixture was designed for serum-free growth of Chinese hamster ovary (CHO) cells

Manufacturer:  Gibco™ 12390035


Catalog No. 12-390-035

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Requires : Supplementation
This item is not returnable. View return policy



F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.


  • Contains zinc, hypoxanthine, and thymidine
  • Serum-free growth of CHO cells in F-10 has led to variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12)
  • Contains no proteins or growth factors and is modified as follows: with phenol red, HEPES, L-glutamine
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (1.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging


Cell Culture, Mammalian Cell Culture

For research use only.

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Shipping Condition: Room Temperature



Room Temperature
12 Months From Date of Manufacture
primay rat astrocytes
High Glucose
CHO, COS-7, and rat prostate epithilial cells
Ham's F-10 Nutrient Mixture
Ham's F-10 Nutrient Mixture
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
500 ml
Phenol Red
Sustainable packaging
L-Glutamine, HEPES, Phenol Red
Sodium Pyruvate
Animal Origin-Free
Product Certifications


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