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Gibco™ Ham's F-10 Nutrient Mix Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Ham's F-10 Nutrient Mixture was designed for serum-free growth of Chinese hamster ovary cells

Manufacturer:  Gibco™ 11550043

Catalog No. 11-550-043


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Description

Description

F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells. This Ham's F-10 is modified as follows: with phenol red, L-glutamine; without HEPES.

  • Contains zinc, hypoxanthine, and thymidine
  • Serum-free growth of CHO cells in F-10 has led to variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12)
  • Contains no proteins or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (1.2g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Liquid
Sustainable packaging
Storage: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture
Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Lipoic Acid, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Potassium Phosphate monobasic (KH2PO4), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
500mL
Sodium Pyruvate; High Glucose; L-Glutamine; Phenol Red
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