Gibco Ham's F-10 Nutrient Mix
Ham's F-10 Nutrient Mixture was designed for serum-free growth of Chinese hamster ovary cells
Manufacturer: Gibco 11550043
F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells. This Ham's F-10 is modified as follows: with phenol red, L-glutamine; without HEPES.
- Contains zinc, hypoxanthine, and thymidine
- Serum-free growth of CHO cells in F-10 has led to variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12)
- Contains no proteins or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (1.2g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
- Sustainable packaging
Cell Culture, Mammalian Cell Culture
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Shipping Condition: Room Temperature
|Storage: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture|
|Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Lipoic Acid, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Potassium Phosphate monobasic (KH2PO4), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol|
|Sodium Pyruvate; High Glucose; L-Glutamine; Phenol Red|
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