Ham's F-12 Nutrient Mix Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Designed for serum-free, single-cell plating of Chinese hamster ovary (CHO) cells

Manufacturer: Gibco 11765062

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Catalog No. 11-765-062

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Required Accessories : Supplementation


    Format Liquid
    Formulation Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (anhydrous), Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
    Packaging Sustainable packaging
    Quantity 10 x 500mL
    Storage Requirements Storage: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture
    With Additives Sodium Pyruvate; L-Glutamine; Phenol Red

    F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. This Ham's F-12 is modified as follows: with L-glutamine, phenol red.

    • Contains zinc, putrescine, hypoxanthine, and thymidine
    • Contains no proteins or growth factors
    • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
    • Uses sodium bicarbonate buffer system (1.176g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
    • Sustainable packaging

    Cell Culture, Mammalian Cell Culture

    This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

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