Gibco™ Ham's F-12 Nutrient Mix

Description

Requires

Supplementation

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

With: L-glutamine, Phenol Red

The complete formulation is available.

Using F-12

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Line: CHO, COS-7, and rat prostate epithelial cells
• Cell Type: Primary Rat Astrocytes
• Classification: Animal Origin-free
• Concentration: 1 X
• Form: Liquid
• Product Type: Ham's F-12 Nutrient Mixture
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: Glutamine, Phenol Red, Sodium Pyruvate
• Without Additives: No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco™
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2°C to 8°C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

Safety and Handling

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