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Gibco™ Ham's F-12 Nutrient Mix, powder
Description
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: Sodium Bicarbonate
The complete formulation is available.
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.
Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.
Order Info
Shipping Condition: Room Temperature
Compliance
Gibco F-12 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
Specifications
| Cell Type | Primary Rat Astrocytes |
| Form | Powder |
| Product Type | Ham's F-12 Nutrient Mixture |
| With Additives | Glutamine, Phenol Red, Sodium Pyruvate |
| Without Additives | No HEPES, No Sodium Bicarbonate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco™ |
| Quantity | 50 L |
| Shipping Condition | Room Temperature |
| Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
Safety and Handling
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
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