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Gibco™ Ham's F-12K (Kaighn's) Medium
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Catalog No. 21127030
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21-127-030 10 x 500 mL
21-127-022 500 mL
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Description

Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

This F-12K (Kaighn's) Medium is manufactured as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Using Ham's F-12K

Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Human Hepatocytes
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type Ham's F-12K (Kaighn's) Medium
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 10 x 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
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For Research Use Only. Not for use in diagnostic procedures.

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