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Gibco™ McCoy's 5A (Modified) Medium
Description
Requires
Supplementation
McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:
With: High Glucose, L-glutamine, Bacto-peptone, Phenol Red
Without: Sodium Pyruvate, HEPES
The complete formulation is available.
Using McCoy's 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
- Environmental benefits include:
- Recyclable
Specifications
Specifications
| Cell Line | Rat fibroblasts |
| Cell Type | Biopsy Tissues |
| Classification | Animal Origin |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | McCoy's 5A Modified Medium |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | High Glucose, Glutamine, Phenol Red, Sodium Bicarbonate |
| Without Additives | No HEPES, No Sodium Pyruvate |
| Show More |
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Certificates
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 2645334 | Certificate of Analysis | 01/29/2025 | 16600082 |
| 2754873 | Certificate of Analysis | 01/28/2025 | 16600082 |
| 2867201 | Certificate of Analysis | 01/26/2025 | 16600082 |
| 2935190 | Certificate of Analysis | 01/26/2025 | 16600082 |
| 2935193 | Certificate of Analysis | 01/26/2025 | 16600082 |
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