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Gibco™ McCoy's 5A (Modified) Medium, HEPES Non-distribution product as customer accommodation. Greener Choice Available on GSA/VA Contract for Federal Government customers only.

General-purpose medium that supports propagation of many types of primary cells, established cell lines and explants from biopsy tissues

Manufacturer:  Gibco™ 12330031

Catalog No. 12-330-031

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Requires : Supplementation
This item is not returnable. View return policy



This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

  • Contains reducing agent glutathione, bacto-peptone, and high level of glucose
  • It is modified as follows: with high glucose, L-glutamine, bacto-peptone, phenol red, HEPES; without sodium pyruvate.
  • Includes Dr. Hsu's addition of Hank's salts to enable use outside CO2 incubator
  • Requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses a sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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Shipping Condition: Room Temperature



500 ml
Phenol Red
No Sodium Pyruvate
Room Temperature
Sustainable packaging
Safety and Handling

Safety and Handling

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Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use. For human ex vivo tissue and cell culture processing applications.