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Gibco™ McCoy's 5A (Modified) Medium, HEPES

General-purpose medium that supports propagation of many types of primary cells, established cell lines and explants from biopsy tissues

Manufacturer:  Gibco™ 12330031


Catalog No. 12-330-031

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Requires : Supplementation
This item is not returnable. View return policy



This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.


  • Contains reducing agent glutathione, bacto-peptone, and high level of glucose
  • It is modified as follows: with high glucose, L-glutamine, bacto-peptone, phenol red, HEPES; without sodium pyruvate.
  • Includes Dr. Hsu's addition of Hank's salts to enable use outside CO2 incubator
  • Requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses a sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging
  • Cell Culture, Mammalian Cell Culture
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Order Info

Shipping Condition: Room Temperature



Room Temperature
12 Months From Date of Manufacture
biopsy tissues
Sodium Pyruvate
No Sodium Pyruvate
Animal Origin
McCoy's 5A Modified Medium
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
500 ml
Phenol Red
Sustainable packaging
High Glucose, L-Glutamine, Phenol Red, HEPES, Bacto-peptone
High Glucose
Rat fibroblasts
McCoy's 5A Modified Medium
Product Certifications


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Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For human ex vivo tissue and cell culture processing applications.