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Gibco™ Medium 199, Hanks' Balanced Salts

Originally developed for nutritional studies of chick embryo fibroblasts

Manufacturer:  Gibco™ 12350039


Catalog No. 12-350-039

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Requires : Supplementation
This item is not returnable. View return policy



Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.

  • Contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins
  • It is modified as follows: with Hank's salts, L-glutamine, HEPES, phenol red
  • Formulation contains Hank's salts for use without CO2
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Does not require CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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Shipping Condition: Room Temperature



Room Temperature
12 Months From Date of Manufacture
chick embryo fibroblasts, mouse pancreatic epithelium, and rat lens tissues
Low Glucose
Rat epithelial
Medium 199
Medium 199
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
500 ml
Phenol Red
Sustainable packaging
L-Glutamine, HEPES, Phenol Red, Hanks' Salts
No Sodium Pyruvate
Animal Origin
Product Certifications


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For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.