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Gibco™ Medium 199, HEPES

Originally developed for nutritional studies of chick embryo fibroblasts

Manufacturer:  Gibco™ 12340030


Catalog No. 12-340-030

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Medium 199 broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.


  • Contains adenine, adenosine, hypoxanthine, thymine, and additional vitamins
  • Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
  • It is modified as follows: with phenol red, HEPES, L-glutamine
  • Contains no proteins, lipids, or growth factors
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging
  • Related applications: Cell Culture, Mammalian Cell Culture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals. 

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Shipping Condition: Room Temperature



Room Temperature
12 Months From Date of Manufacture
chick embryo fibroblasts, mouse pancreatic epithelium, and rat lens tissues
Low Glucose
Rat epithelial
Medium 199
Medium 199
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
500 ml
Phenol Red
Sustainable packaging
L-Glutamine, HEPES, Phenol Red
No Sodium Pyruvate
Animal Origin
Product Certifications


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Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.