Gibco Medium 199, No Phenol Red, Sodium Bicarbonate Buffer
Manufacturer: Gibco 11043023
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.
- It is modified as follows: with L-glutamine; without phenol red, HEPES
- Contains adenine, adenosine, hypoxanthine, thymine, and additional vitamins
- Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
- Contains no proteins, lipids, or growth factors
- Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
- Sustainable packaging
Cell Culture, Mammalian Cell Culture
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Shipping Condition: Room Temperature
Gibco Medium 199 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
|2-deoxy-D-ribose, Adenine sulfate, Adenosine 5-phosphate, Adenosine 5-triphosphate, Alpha-tocopherol Phosphate, Ascorbic Acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Cholesterol, Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glutathione (reduced), Glycine, Guanine hydrochloride, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Menadione (Vitamin K3), Niacinamide, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Potassium Chloride (KCl), Pyridoxal hydrochloride, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4) anhydrous, Thiamine hydrochloride, Vitamin A (acetate), Vitamin D2 (Calciferol), i-Inositol|
|7.1 - 7.4|
|Storage: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture|
|No Sodium Pyruvate; L-Glutamine; No Phenol Red|
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For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.