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Gibco™ MEM, autoclavable, no glutamine, powder
Description
Requires
Supplementation
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
With: Phenol Red, Earle's salts
Without: L-glutamine, HEPES, Sodium Bicarbonate
The complete formulation is available.
Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. To allow autoclaving this medium, choline chloride is substituted with choline bitartrate, phenol red is reduced, and succinic acid is added to prevent precipitation at low pH. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
This autoclavable form of Gibco™ cell culture medium requires pH adjustment to 4.1–4.2 before autoclaving, following by sodium bicarbonate and L-glutamine supplementation and final pH adjustment to 7.2–7.4 (see protocol for details).
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
| Cell Type | Primary Rat Astrocytes |
| Classification | Animal Origin-free |
| Form | Powder |
| Product Type | MEM (Minimum Essential Medium) |
| Serum Level | Standard Serum Supplementation |
| Sterility | Sterile-filtered |
| With Additives | Phenol Red, Earle's Balanced Salt Solution (EBSS) |
| Without Additives | No HEPES, No Sodium Bicarbonate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
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Safety and Handling
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
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