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Gibco™ MEM, high glucose, high sodium bicarbonate
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Catalog No. A1451801
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Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using our media selector tool.

This MEM is modified as follows:

With: Phenol Red, D-glucose (6 g/L), Sodium Bicarbonate (3.7 g/L)

Without: L-glutamine, HEPES

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Product Intended Use

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Certifications

ISO 13485

TRUSTED_SUSTAINABILITY

Specifications

Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type Primary Rat Astrocytes
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type MEM (Minimum Essential Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives High Glucose, Phenol Red, Sodium Bicarbonate
Without Additives No Glutamine, No HEPES, No Sodium Pyruvate
For Use With (Application) Mammalian Cell Culture
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Regulatory Status For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Content And Storage Storage conditions: 2-8 Deg;C. Protect from light



Shipping conditions: Ambient



Shelf life: 12 months from date of manufacture
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