Gibco™ MEM α, nucleosides

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

Supplier:  Gibco™ 12571063

Catalog No. 12-571-063

Description

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:
With: Ribonucleosides, Deoxyribonucleosides, Phenol Red, L-glutamine

The complete formulation is available.

Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Rat Astrocytes
• Concentration: 1 X
• Product Type: MEM α (Minimum Essential Medium α)
• Sterility: Sterile-filtered
• Without Additives: No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 500 mL

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
• Green Features: Sustainable packaging
• Product Line: Gibco™
• Shipping Condition: Room Temperature

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.