Gibco™ MEM α, nucleosides, no ascorbic acid

Description

Requires

Supplementation

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With: Deoxyribonucleosides, Ribonucleosides, L-glutamine, Phenol Red

Without: Ascorbic acid

The complete formulation is available.

Using MEM α

MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This MEM is modified without ascorbic acid. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

MEM α is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Cell Type: Primary Rat Astrocytes
• Classification: Animal Origin-free
• Concentration: 1 X
• Form: Liquid
• Product Type: MEM α (Minimum Essential Medium α)
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Deoxyribonucleosides, Ribonucleosides
• Without Additives: No Ascorbic Acid
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco™
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2 – 8°C. Protect from light.
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

For Research Use Only. Not for use in diagnostic procedures.