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Gibco™ MEM (Richter's modification), no phenol red

Minimum Essential Medium is one of the most commonly used of all cell culture media

Manufacturer:  Gibco™ 10373017

Encompass

Catalog No. 10-373-017


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Description

Description

MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification.

  • Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • It is modified as follows: with L-glutamine, Gentamycin, Proline, sodium pyruvate, zinc; without HEPES, phenol red and insulin
  • Modified formulation of Eagle's MEM that contains iron, zinc, putrescine, fatty acids, and gentamycin, high concentrations of both choline chloride and i-Inositol
  • Made with Earle's salts
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Specifications

1X
Sterile-filtered
Room Temperature
Gibco™
L-Glutamine
12 Months From Date of Manufacture
pirmary rat astrocytes
HEPES, Phenol Red, Insulin
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
MEM (Minimum Essential Medium)
MEM (Minimum Essential Medium)
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
500 ml
Liquid
No Phenol Red
Sustainable packaging
L-Glutamine, Gentamycin, Proline, Sodium Pyruvate, Zinc
Sodium Pyruvate
Animal Origin-Free
No HEPES
SDS
Documents
Product Certifications
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For Research Use Only. Not for use in diagnostic procedures.