Gibco™ MEM (Richter's Modification), No Phenol Red   

Minimum Essential Medium is one of the most commonly used of all cell culture media

Manufacturer:  Gibco™ 10373017

Catalog No. 10-373-017

Description

MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification.

• Available with Earle's salts for use in CO₂ incubator, or with Hank's salts for use without CO₂
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO₂ environment to maintain physiological pH
• It is modified as follows: with L-glutamine, Gentamycin, Proline, sodium pyruvate, zinc; without HEPES, phenol red and insulin
• Modified formulation of Eagle's MEM that contains iron, zinc, putrescine, fatty acids, and gentamycin, high concentrations of both choline chloride and i-Inositol
• Made with Earle's salts
• Sustainable packaging

Cell Culture, Mammalian Cell Culture

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Order Info

Shipping Condition: Room Temperature

Compliance

Gibco MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Format: Liquid
• Packaging: Sustainable packaging
• Storage Requirements: Storage: 2-8°C. Protect from light. Shelf life : 12 months from date of manufacture

• Formulation: Biotin, Calcium Chloride (CaCl₂) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Chloride 6H₂O, Folinic Acid Calcium salt, Gentimicin Sulfate, L-Arginine hydrochloride, L-Asparagine-H₂O, L-Cystine, L-Glutamine, L-Histidine hydrochloride-H₂O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO₄) (anhyd.), Niacinamide, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxal hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO₃), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH₂PO₄) anhydrous, Sodium Pyruvate, Thiamine hydrochloride, Vitamin B12, Zinc sulfate (ZnSO₄-7H₂O), i-Inositol
• Quantity: 500mL
• With Additives: Sodium Pyruvate; L-Glutamine; No Phenol Red

For Research Use Only. Not for use in diagnostic procedures.