Gibco™ MEM, suspension, no calcium, no glutamine

Description

Requires

Supplementation

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: Earle's salts, Phenol Red

Without: L-glutamine, HEPES, Calcium

The complete formulation is available.

MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. This MEM formulation is made without calcium for use with suspension cultures. This MEM formulation contains Earle's salts for use in a CO₂ incubator. This product is made with Earle's salts.

Product Intended Use

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Cell Type: Primary Rat Astrocytes
• Classification: Animal Origin-free
• Concentration: 1 X
• Form: Liquid
• Product Type: MEM (Minimum Essential Medium)
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• With Additives: Low Glucose, Phenol Red, Earle's Balanced Salt Solution (EBSS)
• Without Additives: No Glutamine, No HEPES, No Sodium Pyruvate, No Calcium
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco™
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2-8°C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.