Gibco MEM, Suspension, No Calcium, No Glutamine
Minimum Essential Medium is one of the most commonly used of all cell culture media
Manufacturer: Gibco 11380037
MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification.
- Modified as follows: with Earle's salts, phenol red; without L-glutamine, HEPES, Calcium
- Formulation is made without calcium for use with suspension cultures
- Contains Earle's salts for use in CO2 incubator
- Made with Earle's salts
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
- Sustainable packaging
Cell Culture, Mammalian Cell Culture
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Shipping Condition: Room Temperature
|Storage: 2-8°C. Protect from light. Shelf life : 12 months from date of manufacture|
|Choline chloride, D-Calcium pantothenate, Folic Acid, L-Arginine hydrochloride, L-Cystine 2HCl, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Potassium Chloride (KCl), Pyridoxal hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol|
|No Sodium Pyruvate; Low Glucose; No Glutamine; Phenol Red|
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Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.