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Gibco™ MEM, suspension, no calcium, no glutamine

Minimum Essential Medium is one of the most commonly used of all cell culture media

Supplier:  Gibco™ 11380037

Encompass_Preferred

Catalog No. 11-380-037


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Requires : Supplementation
This item is not returnable. View return policy

Description

Description

MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification.

  • Modified as follows: with Earle's salts, phenol red; without L-glutamine, HEPES, Calcium
  • Formulation is made without calcium for use with suspension cultures
  • Contains Earle's salts for use in CO2 incubator
  • Made with Earle's salts
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Rat Astrocytes
1 X
MEM (Minimum Essential Medium)
12 Months From Date of Manufacture
Low Glucose, Phenol Red, Earle's Salts
Sustainable packaging
500 mL
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Animal Origin-Free
Liquid
Standard Serum Supplementation
Sterile-filtered
No Glutamine, No HEPES, No Sodium Pyruvate, No Calcium
Gibco™
Room Temperature
SDS
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Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.