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Gibco™ MEM, suspension, no calcium, no glutamine

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Supplier:  Gibco™ 11380037

Encompass_Preferred

Catalog No. 11-380-037


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Requires : Supplementation
This item is not returnable. View return policy

Description

Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:
With: Earle's salts, Phenol Red
Without: L-glutamine, HEPES, Calcium

The complete formulation is available.

MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. This MEM formulation is made without calcium for use with suspension cultures. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Rat Astrocytes
1 X
MEM (Minimum Essential Medium)
Sterile-filtered
No Glutamine, No HEPES, No Sodium Pyruvate, No Calcium
cGMP-compliant under the ISO 13485 standard
500 mL
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Animal Origin-free
Liquid
Standard Serum Supplementation
Low Glucose, Phenol Red, Earle's Balanced Salt Solution (EBSS)
Sustainable packaging
Gibco™
Room Temperature
Safety and Handling

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

SDS
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Documents

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Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.