Gibco™ MEM (Temin's modification) (2X), no phenol red

Catalog No.	Quantity
11-935-046	500 mL

Catalog No. 11-935-046 Supplier Gibco™ Supplier No. 11935046

Description

Requires

Supplementation

Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media.

Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: L-glutamine, Earle's salts, Sodium Bicarbonate

Without: HEPES, Phenol Red

The complete formulation is available.

MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. Temin's Modification is a 2x MEM with 4x the vitamins and amino acids of the original MEM. This MEM formulation contains Earle's salts for use in a CO₂ incubator. This product is made with Earle's salts.

Product Intended Use

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (8.4 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Cell Type: Primary Rat Astrocytes
• Classification: Animal Origin-free
• Concentration: 1 X
• Form: Liquid
• Product Type: MEM (Minimum Essential Medium)
• Sterility: Sterile-filtered
• With Additives: High Glucose, Glutamine, Earle's Balanced Salt Solution (EBSS), Sodium Bicarbonate
• Without Additives: No HEPES, No Phenol Red, No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2-8°C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.