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Gibco™ MEM (Temin's Modification) (2X), No Phenol Red Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media

Manufacturer:  Gibco™ 11935046

Catalog No. 11-935-046


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Description

Description

Temin's Modified Eagle Medium (MEM) can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.

  • Modified as follows: with L-glutamine, Earle's salts, sodium bicarbonate; without HEPES, phenol red
  • Temin's Modification is 2x MEM with 4x vitamins and amino acids of original MEM
  • Formulation contains Earle's salts for use in a CO2 incubator
  • Made with Earle's salts
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (8.4g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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Order Info

Shipping Condition: Room Temperature

Compliance

Gibco MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Specifications

Liquid
Sustainable packaging
Storage: 2-8°C. Protect from light. Shelf life : 12 months from date of manufacture
Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, L-Arginine hydrochloride, L-Cystine, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Potassium Chloride (KCl), Pyridoxal hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol
500mL
No Sodium Pyruvate; High Glucose; L-Glutamine; No Phenol Red
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For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.