Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Media
  5. Cell Target Type Cell Culture Media
  6. Neuronal Cell Culture Media
  7. A3582901

Gibco™ Neurobasal™ Plus Medium
GREENER_CHOICE

Catalog No. A3582901
Encompass_Preferred
Change view
Click to view available options
Quantity:
500 mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
A3582901 500 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. A3582901 Supplier Gibco™ Supplier No. A3582901
Only null left
Add to Cart
Edge
Add to Cart

Description

Neurobasal Plus Medium is a neuronal basal medium that provides the highest rates of in vitro survival of primary rodent and human stem cell-derived neurons when used with B-27 Plus Supplement. Neurobasal Plus Medium features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rates of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.

Neurobasal Plus Medium, when combined with B-27 Plus Supplement, offers:

  • At least 50% higher survival rate of primary rat neurons compared to classic Neurobasal Medium and B-27 Supplement
  • Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
  • Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
  • Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Available as a 500 mL basal medium, Neurobasal Plus Medium can seamLessly replace other neuronal cell culture media, such as classic Neurobasal Medium. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Neural Cells
Classification Serum-free
Form Liquid
Product Type Neurobasal Medium
With Additives Phenol Red
Product Line Neurobasal
Quantity 500 mL
Shipping Condition Ambient
Tested For Endotoxin, Performance, Sterility, Osmolality, pH
Content And Storage Store in refrigerator (2–8°C) and protect from light.
Culture Type Neuronal Cell Culture
Show More Show Less

Frequently Asked Questions (FAQs)

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?

We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.
What is the osmolality of Neurobasal Plus Medium?

The osmolality is in the range of 215-235 mOsm/kg. The exact osmolality for your lot of the medium can be found in the Certificate of Analysis for that lot number.
I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.
Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.
How do I thaw B-27 Supplements?

We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.
Can I use B-27 Plus Neuronal Culture System to culture non-neuronal cells?

The B-27 Plus Neuronal Culture System was designed for culturing neuronal cells. We have not tested it in other cells.
I am planning to use the B-27 Plus Neuronal Culture System. How can I control the number of glial cells in my neuronal culture?

Add CultureOne Supplement at day 0 with the B-27 Plus Neuronal Culture System to fully suppress both astrocytes and oligodendrocytes with no detrimental effect on neurons. Delaying the addition of CultureOne Supplement to later time points results in increasing levels of astrocytes. Refer to the CultureOne Supplement User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016204_CultureOneSupplement_UG.pdf) for more information.
Do I have to use B-27 Plus Supplement with Neurobasal Plus Medium?

B-27 Plus Supplement and Neurobasal Plus Medium have been designed to work synergistically together for maximal performance. They can be used with other basal media or supplements if necessary.
How long can I maintain my neurons in culture with the B-27 Plus Neuronal Culture System?

We have maintained primary rat cortical neurons up to 8 weeks and rat hippocampal neurons up to 4 weeks. Mouse cortical neurons were maintained for 4 weeks. Human iPSC-derived neurons that have been differentiated from neural stem cells (NSCs) for 9 days have been maintained for 5 weeks. All cells tested with the B-27 Plus System were significantly healthier than cells maintained in culture using the classic products across all time points.
Can I use the B-27 Plus Neuronal Culture System for expansion of proliferating cells, such as neural stem cells (NSCs)?

No, we do not recommend the B-27 Plus System for expanding proliferating cells. We recommend using StemPro NSC SFM, Neural Induction Medium Supplement or B-27 Supplement minus Vitamin A for growing proliferative cells, such as NSCs.
Can I re-freeze B-27 Supplements into smaller aliquots for future use?

Yes, we recommend that you aliquot when you first thaw it and thereafter subject the aliquot to no more than 2 additional freeze/thaw cycles.
How did you upgrade the manufacturing/QC process of the B-27 Plus Supplement and Neurobasal Plus Medium?

We streamlined the manufacturing process, implemented more stringent acceptance criteria and enhanced QC assays for qualification of raw materials and release of finished product to enable better lot-to-lot consistency.
What is the difference between Neurobasal Plus Medium and Neurobasal Medium?

Key amino acids and buffering components have been optimized in Neurobasal Plus Medium. No small molecules or growth factors have been added.

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.