Gibco RPMI 1640 Medium (Dutch Modification), HEPES
Originally developed to culture human leukemic cells in suspension and as monolayer
Manufacturer: Gibco 22409015
Roswell Park Memorial Institute (RPMI) 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. This RPMI is modified as follows: with phenol red, HEPES, low sodium bicarbonate; without L-glutamine.
- Contains reducing agent glutathione and high concentrations of vitamins
- Contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
- Vitamins inositol and choline are present in high concentrations
- Formulation has Dutch modification (addition of HEPES, with sodium bicarbonate lowered to 1g/L)
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (1.0g/L) and therefore requires 5-7% CO2 environment to maintain physiological pH
Cell Culture, Mammalian Cell Culture
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Shipping Condition: Room Temperature
Gibco RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
|No Sodium Pyruvate; No Glutamine; Phenol Red|
|Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, HEPES, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol|
|Storage: 2-8°C. Protect from light. Shelf life: 12 months from date of manufacture|
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For Research Use Only. Not for use in diagnostic procedures.