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Gibco™ RPMI 1640 Medium (Dutch Modification), HEPES Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only. Green Product

Originally developed to culture human leukemic cells in suspension and as monolayer

Manufacturer:  Gibco™ 22409015

Catalog No. 22-409-015


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Description

Description

Roswell Park Memorial Institute (RPMI) 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. This RPMI is modified as follows: with phenol red, HEPES, low sodium bicarbonate; without L-glutamine.

  • Contains reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Formulation has Dutch modification (addition of HEPES, with sodium bicarbonate lowered to 1g/L)
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (1.0g/L) and therefore requires 5-7% CO2 environment to maintain physiological pH

Cell Culture, Mammalian Cell Culture

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Order Info

Shipping Condition: Room Temperature

Compliance

Gibco RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

Specifications

Liquid
500mL
No Sodium Pyruvate; No Glutamine; Phenol Red
Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, HEPES, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Storage: 2-8°C. Protect from light. Shelf life: 12 months from date of manufacture
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For Research Use Only. Not for use in diagnostic procedures.