Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

Catalog No.	Quantity
61-870-036	500 mL
61-870-127	10 x 500 mL

Catalog No. 61-870-036 Supplier Gibco™ Supplier No. 61870036

Description

Requires

Supplementation

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer.

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:

With: GlutaMAX™, Phenol Red

Without: HEPES

The complete formulation is available.

Using RPMI 1640 Medium

RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Line: HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
• Cell Type: Leukemic Cells
• Classification: Animal Origin-free
• Concentration: 1 X
• Form: Liquid
• Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: GlutaMAX, Phenol Red
• Without Additives: No HEPES, No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco, GlutaMAX
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Green Features: ACT Ecolabel (Silver)
• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

Frequently Asked Questions (FAQs)

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.