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Gibco™ RPMI 1640 Medium, No Folate (Folic Acid) Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Originally developed to culture human leukemic cells in suspension and as a monolayer

Manufacturer:  Gibco™ 27016021

Catalog No. 27-016-021


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Description

Description

Roswell Park Memorial Institute (RPMI) 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas.

  • RPMI is modified as follows: with L-glutamine, phenol red; without HEPES, folic acid
  • Gibco RPMI 1640 is unique from other media because it contains reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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Order Info

Shipping Condition: Room Temperature

Compliance

Gibco RPMI 1640 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Specifications

Liquid
Sustainable packaging
Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Glutathione (reduced), Glycine, Hydroxy L-proline, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4-7H2O), Thiamine hydrochloride, Vitamin B12, i-Inositol
500mL
No Sodium Pyruvate; L-Glutamine; Phenol Red
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For Research Use Only. Not for use in diagnostic procedures.