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Gibco™ RPMI 1640 Medium, no folic acid
Originally developed to culture human leukemic cells in suspension and as a monolayer
Supplier: Gibco™ 27016021
Requires : Supplementation
Description
Roswell Park Memorial Institute (RPMI) 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas.
- RPMI is modified as follows: with L-glutamine, phenol red; without HEPES, folic acid
- Gibco RPMI 1640 is unique from other media because it contains reducing agent glutathione and high concentrations of vitamins
- Contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
- Vitamins inositol and choline are present in high concentrations
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (2.0g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH
- Sustainable packaging
Cell Culture, Mammalian Cell Culture
Order Info
Shipping Condition: Room Temperature
Compliance
Gibco RPMI 1640 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Leukemic Cells | |
| 1 X | |
| RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) | |
| Sterile-filtered | |
| No HEPES, No Sodium Pyruvate, No Folic Acid | |
| Gibco™ |
| HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| Glutamine, Phenol Red | |
| Sustainable packaging | |
| Room Temperature |
Safety and Handling
ShelfLife : 12 Months From Date of Manufacture
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Product Title
For Research Use Only. Not for use in diagnostic procedures.