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Gibco RPMI 1640 Medium, No Glucose
Originally developed to culture human leukemic cells in suspension and as a monolayer
Manufacturer: Gibco 11879020
Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Life Technologies offers a variety of RPMI 1640 Medium modifications for a range of cell culture applications.
- Modified as follows: with L-glutamine, phenol red; without glucose, HEPES
- Contains reducing agent glutathione and high concentrations of vitamins
- Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
- Vitamins inositol and choline are present in high concentrations
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
- Sustainable packaging
Cell Culture, Mammalian Cell Culture
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Shipping Condition: Room Temperature
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
|Storage: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture|
|Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, Folic Acid, Glutathione (reduced), Glycine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4-7H2O), Thiamine hydrochloride, Vitamin B12, i-Inositol|
|No Sodium Pyruvate; L-Glutamine; Phenol Red|
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For Research Use Only. Not for use in diagnostic procedures.