Gibco™ StemFlex™ Medium

Description

StemFlex Medium enables long term feeder-free culture of pluripotent stem cells (PSCs) and is optimized to support challenging applications, including single-cell passaging, gene editing, and reprogramming.

StemFlex Medium's unique formulation supports robust expansion of PSCs while offering the convenience of a flexible feeding schedule (including weekend-free options) and the ability to choose the matrix and passaging reagent that best suits specific applications. StemFlex Medium enables the long term feeder-free culture of PSCs without karyotypic abnormalities and maintains the cells' ability to differentiate into all three germ layers.

StemFlex Medium:

• Delivers superior results in challenging applications
• Offers flexibility for PSC culture conditions and feeding schedules
• Provides exceptional value and performance

Superior results

StemFlex Medium is formulated to deliver superior outcomes in challenging applications that stress PSC cultures, such as reprogramming, single cell passaging, and gene editing. It provides superior cell survival compared to a leading competitor (mTeSR1), allowing for greater success in the most relevant cell types.

Flexibility

StemFlex Medium allows for unprecedented three-way usage flexibility for PSC culture conditions: the ability to select an optimal feeding schedule, including a weekend-free option; a choice of matrices, depending upon the needs of the workflow; and the option to select the passaging reagent for the level of cell dissociation required.

Exceptional value and performance

StemFlex Medium is provided in a convenient two-component kit (450 mL basal medium & 50 mL supplement) and, when used with Geltrex Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix, provides a cost-effective, robust system for superior feeder-free culture of human PSCs. StemFlex Medium's ability to enable weekend-free culture of PSCs provides unmatched value among the robust feeder-fee PSC media on the market today.

Specifications

• Cell Line: Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
• Cell Type: Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
• Classification: Animal Origin-free
• Concentration: StemFlex Basal Medium (1X); StemFlex Supplement (10X)
• Form: Liquid
• Product Type: Stem Cell Media
• Serum Level: Serum-free
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: Phenol Red
• Manufacturing Quality: cGMP for medical devices, 21 CFR Part 820 and ISO 13485
• Product Line: StemFlex™
• Quantity: 500 mL
• Shipping Condition: Supplement ships on dry ice, basal can ship at RT
• Content And Storage: • 450 mL Basal Medium, store at 2–8°C and protect from light
  • 50 mL Supplement, store at -5 to -20°C and protect from light
• Culture Type: Adherent Cell Culture, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)

For Research Use Only. Not for use in diagnostic procedures.