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Gibco™ Streptomycin Sulfate
Description
Streptomycin Sulfate acts by binding to 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Streptomycin Sulfate is highly active against gram-negative with some activity against gram-positive bacteria.
- Available as 100g powder
- Used alone or in combination with penicillin for prevention of bacterial contamination of cell cultures
- Recommended working concentration ranges from 50 to 100μg/mL
Cloning, Transformation
Order Info
Shipping Condition: Room Temperature
Compliance
Manufactured at a cGMP compliant facility, which is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
Specifications
| Content And Storage | Storage conditions: 2 to 8°C Shipping conditions: Ambient Shelf life: 24 months from date of manufacture |
| Concentration | 50 to 100 μg/mL |
| Culture Type | Mammalian Cell Culture, Insect Cell Culture |
| Form | Powder |
| Product Type | Antibiotic |
| For Use With (Application) | Bacterial Selection |
| Quantity | 100 g |
| Shipping Condition | Room Temperature |
Frequently Asked Questions (FAQs)
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.
1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.
The following is a suggested procedure for determining toxicity levels and decontaminating cultures:
1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.
Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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